Fly-half Flood and centre Tuilagi have not played in the opening two rounds of the Championship due to injury but have been retained in a 22-man squad at England’s training base at Pennyhill Park.
They join club colleagues Dan Cole, Tom Croft, Geoff Parling and Ben Youngs in the England squad, but back-rower Thomas Waldrom has been released to rejoin Tigers in preparation for Saturday’s Aviva Premiership match against Newcastle Falcons at Welford Road.
England head coach Stuart Lancaster said: "We've had some difficult selection decisions with players coming back into the equation. We will look forward to meeting again on Wednesday night with our matchday 22 and finalising preparations for the game."
England squad:
Forwards
Mouritz Botha (Saracens)
Alex Corbisiero (London Irish)
Dan Cole (Leicester Tigers)
Tom Croft (Leicester Tigers)
Phil Dowson (Northampton Saints)
Dylan Hartley (Northampton Saints)
Courtney Lawes (Northampton Saints)
Ben Morgan (Scarlets)
Geoff Parling (Leicester Tigers)
Chris Robshaw (Harlequins)
Matt Stevens (Saracens)
Rob Webber (London Wasps)
Backs
Chris Ashton (Northampton Saints)
Brad Barritt (Saracens)
Lee Dickson (Northampton Saints)
Owen Farrell (Saracens)
Toby Flood (Leicester Tigers)
Ben Foden (Northampton Saints)
Charlie Hodgson (Saracens)
David Strettle (Saracens)
Manusamoa Tuilagi (Leicester Tigers)
Ben Youngs (Leicester Tigers)
The following players return to their clubs on Tuesday evening and be available for the weekend’s Aviva Premiership Round 16:
Chris Brooker (Harlequins)
Mike Brown (Harlequins)
Calum Clark (Northampton Saints)
Paul Doran Jones (Northampton Saints)
Joe Marler (Harlequins)
Tom Palmer (Stade Francais)
Joe Simpson (London Wasps)
Jordan Turner-Hall (Harlequins)
Thomas Waldrom (Leicester Tigers)
