Club captain Murphy has recovered from a knee injury suffered in the draw with Saracens in Round 3, while Deacon returned from injury to play as replacement in last weekend’s win over the Ospreys and is now elevated to a starting place.
Director of rugby Richard Cockerill said: “Geordan and Deacs are two of our most experienced players and two of the leaders in the camp so it is good to have them back in the squad.
“We’ve had a decent start but it is important that we keep our form going, especially with the autumn international period coming up.”
Tigers are without Miles Benjamin, Tom Croft, Brett Deacon, Rob Hawkins, Wessel Jooste, Julian Salvi and Richard Thorpe this week due to injury.
Saturday's game is shown live on Sky Sports.
Leicester Tigers (v Gloucester Rugby, away, Saturday 4.45pm)
15 Geordan Murphy (c)
14 Scott Hamilton
13 Manusamoa Tuilagi
12 Anthony Allen
11 Vereniki Goneva
10 Toby Flood
9 Ben Youngs
1 Logovi’i Mulipola
2 Tom Youngs
3 Dan Cole
4 Louis Deacon
5 Geoff Parling
6 Steve Mafi
7 Thomas Waldrom
8 Jordan Crane
Replacements
16 George Chuter
17 Marcos Ayerza
18 Martin Castrogiovanni
19 Ed Slater
20 Graham Kitchener
21 Sam Harrison
22 George Ford
23 Matt Smith
Freddie Burns is back at fly-half as Nigel Davies makes five changes to his Gloucester line-up.
Burns returns after missing the European outings, while Rob Cook replaces Martyn Thomas at full-back. Nick Wood and Rupert Harden return at prop and Tom Savage replaces the injured Jim Hamilton in the second row.
Gloucester Rugby:
15 Rob Cook
14 Charlie Sharples
13 Mike Tindall (c)
12 Billy Twelvetrees
11 James Simpson-Daniel
10 Freddie Burns
9 Jimmy Cowan
1 Nick Wood
2 Huia Edmonds
3 Rupert Harden
4 Tom Savage
5 Will James
6 Sione Kalamafoni
7 Akapusi Qera
8 Ben Morgan
Replacements
16 Koree Britton
17 Dan Murphy
18 Shaun Knight
19 Peter Buxton
20 Matt Cox
21 Dan Robson
22 Martyn Thomas
23 Shane Monahan
