Kitchener sat out last week’s defeat at Wasps, while Hamilton and Mafi have not played since the Round 2 trip to Bath.
Smith has recovered from a broken thumb and joins the matchday squad for the first time this season.
Hamilton adds experience to the Tigers backline, while Kitchener lines up next to skipper Louis Deacon in the second row. Smith and Mafi are among the replacements.
Kitchener’s return means a move into the back row for Ed Slater, with Jordan Crane recalled at No8.
Director of rugby Richard Cockerill said: “It’s good to get some more bodies back because we’ve been pretty thin in some positions.
“The effort and endeavour were there at Wasps last week but our accuracy was poor and we have to put that right. Quins will come with the attitude to play their game and we have just got to concentrate on what we do.
“The game is pretty much sold-out and we need the supporters right behind us to help us through this difficult period.”
Dan Cole, Toby Flood, and Ben and Tom Youngs are in the England squad for the start of the autumn series, while Geoff Parling suffered a mild concussion in training with the national team this week and sits out the first Test against Australia.
Unavailable due to injury are Anthony Allen, Gonzalo Camacho, Tom Croft, Vereniki Goneva, Rob Hawkins, Ryan Lamb, Scott Steele, Mathew Tait and Manu Tuilagi.
The last 500 remaining tickets for Saturday’s game are available now from the Tigers ticket office. Phone 0844 856 1880 or call in at Welford Road (open until 5pm Friday and from 9am Saturday) or book online at www.leicestertigers.com
Gates will be open from 2pm to allow supporters to watch coverage of England versus Australia throughout the stadium and on the big screen in Final Whistle. And a fireworks display on the pitch will follow the Tigers match.
Leicester Tigers (v Harlequins, Welford Road, Saturday 5.30pm)
15 Niall Morris
14 Scott Hamilton
13 Terrence Hepetema
12 Dan Bowden
11 Adam Thompstone
10 Owen Williams
9 David Mélé
1 Marcos Ayerza
2 Neil Briggs
3 Logovi’i Mulipola
4 Louis Deacon (c)
5 Graham Kitchener
6 Ed Slater
7 Julian Salvi
8 Jordan Crane
Replacements
16 George Chuter
17 Boris Stankovich
18 Fraser Balmain
19 Steve Mafi
20 Thomas Waldrom
21 Sam Harrison
22 Matt Smith
23 Blaine Scully
Harlequins
15 Nick Evans
14 Charlie Walker
13 Matt Hopper
12 Tim Molenaar
11 Ugo Monye
10 Ben Botica
9 Danny Care
1 Mark Lambert
2 Dave Ward
3 Will Collier
4 Nick Easter (c)
5 George Robson
6 Joe Trayfoot
7 Luke Wallace
8 Tom Guest
Replacements
16 Harry Allen
17 Darryl Marfo
18 Nic Mayhew
19 Charlie Matthews
20 Jack Clifford
21 Karl Dickson
22 Tom Casson
23 Sam Smith
