Murphy will reach the landmark when he captains Tigers at the Walkers Stadium in the final game of the Guinness Premiership regular season – a week after he played his 150th league game for the club in the 16-13 win at Saracens.
Tigers need just one point from Saturday’s game against Bristol to guarantee finishing top of the Guinness Premiership.
And it will see Murphy join an illustrious list of Tigers players, including teammates Martin Corry and Ben Kay, who have played 250 times for the club.
Murphy said: “My brother said to me a week or two ago that if I played the next few games it was going to be 250 appearances.
“I was quite shocked. I didn’t realise it was that many.
“It will be a tremendous honour. I look at some of the players who are up there with that many games and there are some fantastic players.
“It is really pleasing. I feel really good and I am really enjoying my rugby this season. I can’t believe there are that many miles on the clock because I feel quite good.”
Murphy has captained Tigers in the last three games – the Heineken Cup quarter-final victory over Bath Rugby and the Guinness Premiership wins against Sale Sharks and Saracens – in the absence of Martin Corry and Aaron Mauger, and will continue in the role against Bristol.
He said: “It is a tremendous honour but there is so much experience in the team there is not a huge amount to do other than take the toss.”
Tickets for the Bristol game at the Walkers Stadium are available in person from the Tigers ticket office at Welford Road or by calling 0844 856 1880.