Ben Youngs and George Ford will start for England against France this weekend, with Leicester Tigers team-mate Ellis Genge also included in the matchday squad.
England host Les Bleus at Twickenham Stadium on Saturday (4.45pm), with Youngs in line for a 108th cap and Ford moving on to 76.
Eddie Jones said: “We are, of course, disappointed that we can’t defend the championship, but that makes this an even more important game. We want to show what we are capable of.”
England versus France is live on ITV 1 and BBC Radio 5 Live.
The team's final game in the Guinness Six Nations is against Ireland in Dublin next Saturday.
15 Max Malins (Bristol Bears, 6 caps)
14 Anthony Watson (Bath Rugby, 49 caps)
13 Henry Slade (Exeter Chiefs, 37 caps)
12 Owen Farrell (Saracens, 91 caps) (C)
11 Jonny May (Gloucester Rugby, 64 caps)
10 George Ford (Leicester Tigers, 75 caps)
9 Ben Youngs (Leicester Tigers, 107 caps)
1 Mako Vunipola (Saracens, 65 caps)
2 Luke Cowan-Dickie (Exeter Chiefs, 29 caps)
3. Kyle Sinckler (Bristol Bears, 42 caps)
4 Maro Itoje (Saracens, 46 caps)
5 Charlie Ewels (Bath Rugby, 19 caps)
6 Mark Wilson (Newcastle Falcons, 21 caps)
7 Tom Curry (Sale Sharks, 31 caps)
8 Billy Vunipola (Saracens, 59 caps)
16 Jamie George (Saracens, 57 caps)
17 Ellis Genge (Leicester Tigers, 26 caps)
18 Will Stuart (Bath Rugby, 10 caps)
19 Jonny Hill (Exeter Chiefs, 7 caps)
20 Ben Earl (Bristol Bears, 11 caps)
21 Dan Robson (Wasps, 10 caps)
22 Ollie Lawrence (Worcester Warriors, 4 caps)
23 Elliot Daly (Saracens, 50 caps)