Leicester Tigers player Matt Riddington is included among the 23-man squad.
The talent identification process began back in March 2013 with each of the four divisions (London and the South East, Midlands, North, and South West) naming a squad of 25 in December.
Over the last week the players have been given an opportunity to impress the coaching team during two days of competition.
Head coach Steve Shortland said: “It is a huge honour to pull on a shirt with the rose, and the performances and level of commitment shown by players this week, have proved how much it means to them to represent their country at this festival.
“It is exciting to see how much talent there is in this age group and how individuals have developed as a result of this week’s experience.”
England play a second fixture against Wales at Neath RFC on April 27.
England U16s (School, Club, Academy, County)
Reuben Bird-Tulloch (Eltham College, Blackheath RFC, Saracens, Kent)
George Cave (Bedford School, Bedford Blues RFC, Northampton Saints, East Midlands)
Alistair Crossdale (Altrincham GS, Bowdon RFC, Sale Sharks, Cheshire)
Thomas Curry (Bishop Heber HS, Crewe & Nantwich RFC, Sale Sharks, Cheshire)
Ben Earl (Tonbridge School, Sevenoaks RFC, Saracens, Kent)
Charlie Gowling (Sedbergh School, Newcastle Falcons, CND)
Oliver Graham (Castle School Taunton, Taunton RFC, Bath Rugby, Somerset)
Kalius Hutchinson (Coundon Court, Old Coventrians, Worcester Warriors, Warwickshire)
Gabriel Ibitoye (Trinity School, Harlequins, Surrey)
Nick Isiekwe (Nicholas Breakspear School, Old Albanians RFC, Saracens, Hertfordshire)
Ben Kelland (Royal Hospital School, Colchester RFC, Northampton Saints, Eastern Counties)
Matt Kouris (Wavell School Farnborough, Basingstoke RFC, London Irish, Hampshire)
Suva Ma'asi (Wootton School, Ampthill RFC, Northampton Saints, East Midlands)
Joseph Morris (King Edward VI Aston School, Sutton Coldfield RFC, Worcester Warriors, North Midlands)
Jack Nay (The Hayesbrook School, Saracens, Kent)
Joshua Nott (RGS Worcester, Worcester RFC, Worcester Warriors, North Midlands)
Matt Riddington (Oakham School, Leicester Tigers, Leicestershire)
Callum Sirker (Harrow School, Richmond RFC, Wasps, Middlesex)
Marcus Street (Queen Elizabeth's CC, Cullumpton RFC, Exeter Chiefs, Devon)
Will Vaughan (Heathfield Comm. School, Taunton RFC, Bath Rugby, Somerset)
Henry Walker (Bromsgrove School, Spartans RFC, Gloucester, North Midlands)
Matt Williams (Wellington College, London Irish, Berkshire)
Zac Xiourouppa (Ellesmere College, Shrewsbury RFC, Worcester Warriors, North Midlands)