Forward Calum Green and backs Sam Harrison and Will Hurrell have been named in the 32-man group which will compete in the RBS U20 Six Nations campaign in the New Year before heading to the IRB Junior World Championships in Argentina in June.
Head coach Mark Mapletoft said: “We've got a great deal of experience and we're able to add to that with players who have been very successful at Under 18 level over the last two years.
“We also have a lot of raw talent to work with and the Under 18s certainly looked very strong in South Africa this summer.
“We're heading in the right direction and we've established ourselves as the second best side in the world behind New Zealand over the last couple of years.
“The age grade sides do change every year but we continually look at ourselves to keep improving and keep focusing on their development as players and as individuals.”
The Under 20s start their preparations with two days together at Twickenham during September and training camps in December and January before kicking off the Six Nations against Wales on February 5.
England Under 20 EPS
Forwards
Joe Marler (Harlequins)**
Lee Imiolek (Sale Sharks)
Jamie George (Saracens)**
Arthur Ellis (London Wasps)*
Shaun Knight (Gloucester Rugby)**
Mako Vunipola (Bristol Rugby)
Kieran Brookes (Newcastle Falcons)
Calum Green (Leicester Tigers)
Danny Wright (Gloucester Rugby)*
James Gaskell (Sale Sharks)**
Peter Elder (London Wasps)*
Jamie Gibson (London Irish)*
Joe Trayfoot (Harlequins)
Jacob Rowan (Leeds Carnegie)*
Will Welch (Newcastle Falcons)
Alex Gray (Newcastle Falcons)
Jackson Wray (Saracens)
Backs
Sam Harrison (Leicester Tigers)*
Jordi Pasqualin (Gloucester Rugby)*
Sam Stuart (Harlequins)
Freddie Burns (Gloucester Rugby)*
Rory Clegg (Harlequins)**
Jake Sharp (Saracens)
Tom Catterick (Newcastle Falcons)
Tom Casson (Saracens)*
Jonny May (Gloucester Rugby)
Sam Smith (Harlequins)
Christian Wade (London Wasps)
Marcus Watson (London Irish)
Will Hurrell (Leicester Tigers)**
Tom Homer (London Irish)**
Jack Wallace (London Wasps)
**2009 Junior World Cup squad
*Capped in the 2009 RBS U20 Six Nations