Leicester Tigers are guaranteed a home semi-final and could face Bath Rugby, London Wasps or London Irish at Welford Road on Sunday, May 16 (kick off 4pm).
Tigers will discover who they meet in the last four after today's final round of regular season games.
Bath Rugby are in fourth place and host Leeds Carnegie at the Recreation Ground needing three match points to ensure a fourth-place finish.
London Wasps are fifth and travel to Kingston Park to play Newcastle Falcons while sixth-placed London Irish take on Northampton Saints at Madejski Stadium.
The other semi-final will be contested between Northampton Saints and Saracens, with both teams still looking to secure home advantage going into the final round of games. Click here to see the Guinness Premiership table.
Bath Rugby v Leeds Carnegie
Harlequins v Sale Sharks
Leicester Tigers v Saracens
London Irish v Northampton Saints
Newcastle Falcons v London Wasps
Worcester Warriors v Gloucester Rugby
Tickets for Tigers’ Guinness Premiership semi-final are available now on 0844 856 1880, online or in person from the Welford Road ticket office.