Harry Ellis, George Chuter, Louis Deacon and Martin Corry all start, while Julian White and Lewis Moody claim a place on the bench for the Calcutta Cup clash.
Tigers have more players in both the starting XV and the 22 than any other club, with London Wasps next in line with a total of four players making the match-day squad.
Corry , along with Mike Tindall and Phil Vickey, is in line to win his 50th international cap, with Ellis making his first England appearance since last year's 6 Nations tournament.
Tindall also returns to the team for his first international since the 2006 competition and Vickery leads the team for the first time in a full test at Twickenham.
Saracens inside centre Andy Farrell is set for his England debut in a side which also sees the return from test retirement of Jason Robinson and a first international start for Jonny Wilkinson (Newcastle Falcons) since the RWC 2003 final.
Ashton said: "I'm delighted to announce a strong England 22 for what will be a very challenging match against Scotland on Saturday. The starting XV includes a debut for Andy Farrell. It is well documented that I have been an admirer of Andy 's career for a number of years and I'm looking forward to seeing him play rugby union for his country.
"I'm also pleased that Jason Robinson has decided to return from international retirement and that Jonny Wilkinson has returned to fitness after his recent injury and that both are available for the Scotland game."
England
I5 Iain Balshaw Gloucester Rugby
14 Josh Lewsey London Wasps
13 Mike Tindall Gloucester Rugby
12 Andy Farrell Saracens
11 Jason Robinson Sale Sharks
10 Jonny Wilkinson Newcastle Falcons
9 Harry Ellis Leicester Tigers
1 Perry Freshwater Perpignan
2 George Chuter Leicester Tigers
3 Phil Vickery London Wasps, capt
4 Louis Deacon Leicester Tigers
5 Danny Grewcock Bath Rugby
6 Joe Worsley London Wasps
7 Magnus Lund Sale Sharks
8 Martin Corry Leicester Tigers
Replacements
16 Lee Mears Bath Rugby
17 Julian White Leicester Tigers
18 Tom Palmer London Wasps
19 Lewis Moody Leicester Tigers
20 Pete Richards Gloucester Rugby
21 Toby Flood Newcastle Falcons
22 Mathew Tait Newcastle Falcons
