Four Leicester Tigers players remain with the England squad preparing for the final Test of the autumn, against South Africa at Twickenham this weekend.
England head coach Eddie Jones has named a 34-player squad which includes uncapped Leicester hooker Nic Dolly and club colleagues George Martin, Freddie Steward and Ben Youngs.
Tigers captain Ellis Genge was withdrawn from the squad to face Australia last weekend after a positive test for Covid and has been placed in isolation.
Owen Farrell and Jamie George have withdrawn from the squad with injuries picked up in England’s 32-15 win over Australia.
Gloucester front-rowers Harry Elrington and Jack Singleton have been added to the squad and Joe Marler will join them on Friday after completing his 10-day isolation period following his positive COVID test result.
England against South Africa is a 3.15pm kick-off on Saturday with live coverage on Amazon Prime Sport and TalkSPORT.
Jamie Blamire (Newcastle Falcons, 4 caps)
Callum Chick (Newcastle Falcons, 2 caps)
Tom Curry (Sale Sharks, 35 caps)
Trevor Davison (Newcastle Falcons, 2 caps)
Nic Dolly (Leicester Tigers, uncapped)
Alex Dombrandt (Harlequins, 3 caps)
Harry Elrington (Gloucester Rugby, uncapped)
Charlie Ewels (Bath Rugby, 25 caps)
Jonny Hill (Exeter Chiefs, 11 caps)
Maro Itoje (Saracens, 50 caps)
Courtney Lawes (Northampton Saints, 89 caps)
Lewis Ludlam (Northampton Saints, 10 caps)
Joe Marler (Harlequins, 73 caps)
George Martin (Leicester Tigers, 1 cap)
Bevan Rodd (Sale Sharks, 1 cap)
Sam Simmonds (Exeter Chiefs, 8 caps)
Kyle Sinckler (Bristol Bears, 46 caps)
Jack Singleton (Gloucester Rugby, 3 caps)
Will Stuart (Bath Rugby, 14 caps)
Sam Underhill (Bath Rugby, 26 caps)
Mark Atkinson (Gloucester Rugby, 1 cap)
Tommy Freeman (Northampton Saints, uncapped)
George Furbank (Northampton Saints, 5 caps)
Max Malins (Saracens, 9 caps)
Joe Marchant (Harlequins, 6 caps)
Jonny May (Gloucester Rugby, 68 caps)
Alex Mitchell (Northampton Saints, 1 cap)
Raffi Quirke (Sale Sharks, 1 cap)
Adam Radwan (Newcastle Falcons, 2 caps)
Henry Slade (Exeter Chiefs, 42 caps)
Marcus Smith (Harlequins, 4 caps)
Freddie Steward (Leicester Tigers, 4 caps)
Manu Tuilagi (Sale Sharks, 45 caps)
Ben Youngs (Leicester Tigers, 111 caps)