Flanker Mafi and full-back Hamilton both missed the Aviva Premiership wins over Exeter Chiefs and Sale Sharks, but have returned to fitness in time for the European opener in France this weekend.
Loosehead prop Marcos Ayerza is also included in the Tigers squad for the first time this season on his return from international duty with Argentina.
Vereniki Goneva makes his Heineken Cup debut on the wing as two-time European champions Tigers visit four-time winners Toulouse in Pool 2.
Leicester Tigers (vs Toulouse, Heineken Cup, Sunday 4pm local time, 3pm UK time)
15 Scott Hamilton
14 Niall Morris
13 Manusamoa Tuilagi
12 Anthony Allen
11 Vereniki Goneva
10 Toby Flood
9 Ben Youngs
1 Logovi’i Mulipola
2 Tom Youngs
3 Dan Cole
4 Graham Kitchener
5 Geoff Parling
6 Steve Mafi
7 Thomas Waldrom
8 Jordan Crane (c)
Replacements
16 George Chuter
17 Marcos Ayerza
18 Martin Castrogiovanni
19 Ed Slater
20 Richard Thorpe
21 Sam Harrison
22 George Ford
23 Matt Smith
Toulouse: 15 Clément Poitrenaud, 14 Vincent Clerc, 13 Florian Fritz, 12 Gaël Fickou, 11 Yoann Huget, 10 Luke McAlister, 9 Jean-Marc Doussain; 1 Gurthro Steenkamp, 2 Gary Botha, 3 Census Johnston, 4 Romain Millo-Chluski, 5 Yoann Maestri, 6 Yannick Nyanga, 7 Thierry Dusautoir, 8 Louis Picamoles ,
Replacements: 16 Christopher Tolofua, 17 Jean-Baptiste Poux, 18 Antoine Guillamon, 19 Patricio Albacete, 20 Jean Bouilhou, 21 Yannick Jauzion, 22 Luke Burgess, 23 Timoci Matanavou
