Tigers qualified for the last four for a third successive year after topping Pool B thanks to home wins over Cardiff Blues and Sale Sharks, together with a bonus point from defeat at Bath Rugby.
The defending champions now head for Cardiff’s Millennium Stadium for the semi-final and another crunch encounter with London Wasps, the team that eliminated Tigers at that stage two seasons ago and also the victors in last season's Heineken Cup Final.
Wasps secured their place from Pool A with Saturday's win over Newcastle Falcons.
Tigers captain Martin Corry, who lifted the trophy at Twickenham last season, said: “We are obviously delighted to top the pool after three tough games. We are in the semi-finals again and that means another big day at the Millennium Stadium.
“We are the defending champions and we want to keep our grip on the trophy for as long as possible but I’m sure the other three semi-finalists will be thinking they can go all the way too.”
Tigers versus Wasps will kick off at 2.30pm at the Millennium Stadium, with Saracens against The Ospreys in the second semi-final to follow at 5.15pm.
Tickets details will be released on the Tigers website soon.