Tom Youngs, who has been part of the matchday squad in all four games so far, is incouded alongside his brother Ben Youngs, Manu Tuilagi and the uncapped Ed Slater.
The England squad reported to their training base at Pennyhill Park on Monday evening to begin their build-up for the trip to Rome.
England squad
Forwards (16)
David Attwood (Bath Rugby)
Dylan Hartley (Northampton Saints)
Matt Kvesic (Gloucester Rugby)
Tom Johnson (Exeter Chiefs)
Joe Launchbury (London Wasps)
Courtney Lawes (Northampton Saints)
Joe Marler (Harlequins)
Ben Morgan (Gloucester Rugby)
Matt Mullan (London Wasps)
Chris Robshaw (Harlequins)
Ed Slater (Leicester Tigers)
Henry Thomas (Sale Sharks)
Mako Vunipola (Saracens)
David Wilson (Bath Rugby)
Tom Wood (Northampton Saints)
Tom Youngs (Leicester Tigers)
Backs (18)
Chris Ashton (Saracens)
Brad Barritt (Saracens)
Mike Brown (Harlequins)
Freddie Burns (Gloucester Rugby)
Luther Burrell (Northampton Saints)
Danny Care (Harlequins)
Lee Dickson (Northampton Saints)
Kyle Eastmond (Bath Rugby)
Owen Farrell (Saracens)
George Ford (Bath Rugby)
Alex Goode (Saracens)
Jonny May (Gloucester Rugby)
Jack Nowell (Exeter Chiefs)
Manusamoa Tuilagi (Leicester Tigers)
Billy Twelvetrees (Gloucester Rugby)
Anthony Watson (Bath Rugby)
Marland Yarde (London Irish)
Ben Youngs (Leicester Tigers)
