Six Leicester Tigers players remain with England this week in the build-up to Sunday’s first Quilter International against Wales at Twickenham.
Head coach Eddie Jones has called up 33 players who meet today at Pennyhill Park to continue preparations for the opening match of the summer.
Forwards Dan Cole and Ellis Genge are joined by Tigers colleagues George Ford, Jonny May, Manu Tuilagi and Ben Youngs in the squad.
Jones said: “We now move into a four-game preparation phase where the development of the team tactically is paramount.
“By the time we fly out on the 8th of September we will be ready to win the Rugby World Cup. We are not there yet but we have four games to get ready.
“Players excluded from being involved in this camp are naturally disappointed but may get another opportunity so they must be ready."
England play Wales on Sunday and Ireland on August 24 at Twickenham then Italy in Newcastle as part of the Quilter Internationals as well as an away fixture against Wales on August 17.
Dan Cole (Leicester Tigers, 85 caps)
Luke Cowan-Dickie (Exeter Chiefs, 11 caps)
Tom Curry (Sale Sharks, 10 caps)
Charlie Ewels (Bath Rugby, 10 caps)
Ellis Genge (Leicester Tigers, 9 caps)
Jamie George (Saracens, 37 caps)
Maro Itoje (Saracens, 27 caps)
George Kruis (Saracens, 32 caps)
Joe Launchbury (Wasps, 58 caps)
Courtney Lawes (Northampton Saints, 71 caps)
Lewis Ludlam (Northampton Saints, uncapped)
Joe Marler (Harlequins, 59 caps)
Kyle Sinckler (Harlequins, 22 caps)
Jack Singleton (Saracens, uncapped)
Sam Underhill (Bath Rugby, 9 caps)
Billy Vunipola (Saracens, 41 caps)
Mako Vunipola (Saracens, 53 caps)
Harry Williams (Exeter Chiefs, 17 caps)
Mark Wilson (Newcastle Falcons / Sale Sharks, 13 caps)
Joe Cokanasiga (Bath Rugby, 4 caps)
Elliot Daly (Saracens, 30 caps)
Owen Farrell (Saracens, 70 caps)
George Ford (Leicester Tigers, 55 caps)
Piers Francis (Northampton Saints, 4 caps)
Willi Heinz (Gloucester Rugby, uncapped)
Jonathan Joseph (Bath Rugby, 40 caps)
Joe Marchant (Harlequins, uncapped)
Jonny May (Leicester Tigers, 45 caps)
Ruaridh McConnochie (Bath Rugby, uncapped)
Henry Slade (Exeter Chiefs, 22 caps)
Manu Tuilagi (Leicester Tigers, 32 caps)
Anthony Watson (Bath Rugby, 33 caps)
Ben Youngs (Leicester Tigers, 85 caps)