Tim Skepper and 16-year-old Max Bylina jumped 364 feet from a bridge spanning the Zambezi River at the Victoria Falls on the Zimbabwe-Zambia border.
The Tigers supporters raised more than £500 for the Matt Hampson Trust as they experienced the thrill of a lifetime.
Tigers legend Dusty Hare is with the group as they visit Zambia before Saturday’s third Test in Johannesburg.
Hare said: “I am very grateful to them for doing it. They needed plenty of courage, I can tell you!
“People all donated money for them doing it and we thank them for that.”
Skepper said: "Doing a bungee jump in such a fantastic setting as Victoria Falls was a truly amazing experience, although somewhat scary!
“Myself and Max are very pleased with the amount of money we have raised and we would like to thank our fellow Tigers Events travellers for their generosity in raising over £500 for Matt."
Hare, who toured New Zealand with the Lions in 1983, is accompanying the Tigers Events team that has put together a series of tours to South Africa for the Lions tour.
While the Tigers Events packages feature some stunning sightseeing, such as the trip to Victoria Falls, the rugby forms the focal part of the trips, and Hare described the second Test as unforgettable.
Hare said: “The atmosphere in the stadium was fantastic, absolutely fantastic. It was an honour to be there.”
Tigers Events traveller Geoff Hall said: "The last three days in Livingstone have been amazing and the Victoria Falls is simply breathtaking. We are all now hoping for a win in the final Test and hope that Tom Croft and Harry Ellis get through the match injury-free."